膜杰作
Star Staining
分子别名（Synonym）
CC chemokine receptor 8,CD198,ChemR1,CKRL1,CKR-L1,CKRL1,CY6,GPRCL1,GPRCY6,GPR-CY6,TER-1
表达区间及表达系统（Source）
Biotinylated Human CCR8 Protein, His,Flag&Avitag (CC8-H82D4) is expressed from human 293 cells (HEK293). It is a membrane protein, contains complete wild type 7-transmembrane domans (TMD) and extracellular doman (ECD).
Predicted N-terminus: Asp 2
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag, followed by a Flag tag and an Avi tag (Avitag™) at the C-terminus. The protein has a calculated MW of 60.5 kDa.
Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution.
The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein(MSP1D1) upon removal of the detergent.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human CCR8 Protein, His,Flag&Avitag (Cat. No. CC8-H82D4) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-CCR8 Antibody, Human IgG1 with a linear range of 0.3-20 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human CCR8 Protein, His,Flag&Avitag (Cat. No. CC8-H82D4) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-CCR8 Antibody with a linear range of 0.2-5 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Anti-CCR8 Antibody captured on Protein A Chip can bind Biotinylated Human CCR8 Protein, His,Flag&Avitag (Cat. No. CC8-H82D4) with an affinity constant of 124 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
This entry represents CC chemokine receptor 8 (CCR8), which it is expressed predominantly in lymphoid tissues and has also been found in glomerular podocytes and human umbilical vein endothelial cells (HUVECs). CCR8 is associated with Th2 lymphocytes, which are critical for allergy, and has a role in lymphocyte activation, migration, proliferation and differentiation and in allergic diseases. CCR8 binds to CCL1 (also known as I-309) and to CCL16 (also known as liver expressed chemokine). It also exhibits a high affinity for three chemokines of viral origin: vMIP-I, vMIP-II and vMCC-I.
