膜杰作
Star Staining
分子别名（Synonym）
Ig gamma-3 chain C region,IgG,IgG3
表达区间及表达系统（Source）
Biotinylated Human IgG3 Fc Protein, Avitag (IG3-H8213) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 377 (Accession # P01860-1).
Predicted N-terminus: Gly
Request for sequence
蛋白结构（Molecular Characterization）
The protein has a calculated MW of 33.4 kDa. The protein migrates as 38-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human IgG3 Fc Protein, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human IgG3 Fc Protein, Avitag (Cat. No. IG3-H8213) is more than 95% and the molecular weight of this protein is around 65-80 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human CD64, His Tag (Cat. No. FCA-H52H1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IgG3 Fc Protein, Avitag (Cat. No. IG3-H8213) with a linear range of 0.08-2 ng/mL (QC tested).
Protocol
背景（Background）
Immunoglobulin G3 (IgG3) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-3 chain C region (IgG3-Fc / IGHG3) contains two constant regions of IgG3 H chain (CH2, CH3).
