膜杰作
Star Staining
抗体来源（Source）
APC-Labeled Monoclonal Anti-Human 218 linker Antibody, Rabbit IgG (P3C09) is a monoclonal antibody recombinantly expressed from HEK293 cells, which provides higher batch consistency and long term security of supply.
应用（Application）
Flow Cytometry (Evaluation of CAR and SCFV containing 218linker on Human cells).
克隆号（Clone）
P3C09
种属（Species）
Rabbit
亚型（Isotype）
Rabbit IgG | Rabbit Kappa
特异性（Specificity）
This product is a specific antibody specifically reacts with CAR or scFv containing the 218 linker sequence.
种属反应性（Reactivity）
Human
免疫原（Immunogen）
Purified 218 linker protein.
偶联（Conjugate）
APC
Excitation Wavelength: 640 nm
Emission Wavelength: 661 nm
推荐稀释比（Recommended Dilution）
1:50
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 0.2% BSA, 0.03% Proclin300 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
活性（Bioactivity）-FACS
Flow cytometric analysis of Anti-BCMA (C11D5.3) CAR 293 cells staining with APC-Labeled Monoclonal Anti-human 218 Linker Antibody, Rabbit IgG (P3C09) (Cat. No. 218-AHFMY3) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL). APC signal was used to evaluate the binding activity (QC tested).
Protocol
背景（Background）
The whitlow/218 linker, with the sequence GSTSGSGKPGSGEGSTKG, is a commonly used synthetic peptide linker. Whitlow/218 linker often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The scFv containing the 218 linker showed reduced aggregation and was found to be more stable to proteolysis in vitro.
