膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-HRSV Glycoprotein (RSV-G) Antibody, Human IgG1 (8G9) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号（Clone）
8G9
种属（Species）
Mouse
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Virus.
免疫原（Immunogen）
Recombinant HRSV (A) Glycoprotein G Protein is expressed from human 293 cells.
特异性（Specificity）
Specific recognition of HRSV (A) Glycoprotein G Protein.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.1-125 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
交叉验证（Cross Verification）
This product No cross-reactivity in ELISA with
HRSV (B) Glycoprotein G Protein, His Tag (Cat. No. GLG-V5144).
电泳（SDS-PAGE）
Monoclonal Anti-HRSV Glycoprotein (RSV-G) Antibody, Human IgG1 (8G9) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-HRSV Glycoprotein (RSV-G) Antibody, Human IgG1 (8G9) (Cat. No. RSV-MY2232) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized HRSV (A) Glycoprotein G Protein, His Tag (Cat. No. RSG-V5221) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HRSV Glycoprotein (RSV-G) Antibody, Human IgG1 (8G9) (Cat. No. RSV-MY2232) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Immobilized HRSV (A) Glycoprotein G Protein, His Tag (Cat. No. GLG-V5143) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HRSV Glycoprotein (RSV-G) Antibody, Human IgG1 (8G9) (Cat. No. RSV-MY2232) with a linear range of 0.1-4 ng/mL (Routinely tested).
Protocol
背景（Background）
The two major glycoproteins on the surface of the RSV virion, the attachment glycoprotein (G) and the fusion (F) glycoprotein, control the initial phases of infection. The central region of the G protein contains a 13-amino acid highly conserved domain, partially overlapping the cysteine noose domain with 4 cysteines linked 1–4 and 2–3, followed by a highly basic heparin-binding domain (HBD). The HBD is the likely attachment site for heparan sulfate (HS) found on the surface of most cells. A peptide from the G protein HBD (amino acids 184–198) binds efficiently to HEp-2 cells and inhibits RSV infection.
