膜杰作
Star Staining
分子别名（Synonym）
C-C motif chemokine receptor 9,GPR28,CDw199,GPR-9-6,CC-CKR-9
表达区间及表达系统（Source）
Human CCR9 Full Length Protein (VLP) (CC9-H52P3) is expressed from human 293 cells (HEK293). It contains AA Thr 2 - Leu 369 (Accession # P51686-1).
Predicted N-terminus: Asp
蛋白结构（Molecular Characterization）
Virus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrane Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.
The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Supplied as 0.2 μm filtered solution in PBS, Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
活性（Bioactivity）-ELISA
Immobilized Human CCR9 Full Length Protein (VLP) (Cat. No. CC9-H52P3) at 5 μg/mL (100 μL/well) can bind Anti-CCR9 Antibody with a linear range of 0.001-0.25 μg/mL (QC tested).
Protocol
均一性（Identity）-DLS
The mean peak Radius of VLP is 60-85 nm with more than 95% intensity as determined by dynamic light scattering (DLS).
背景（Background）
The protein encoded by this gene is a G protein-coupled receptor with seven transmembrane domains that belongs to the beta chemokine receptor family. Chemokines and their receptors are key regulators of thymocyte migration and maturation in normal and inflammation conditions. This gene is differentially expressed in T lymphocytes of the small intestine and colon, and its interaction with chemokine 25 contributes to intestinal intra-epithelial lymphocyte homing to the small intestine. This suggests a role for this gene in directing immune responses to different segments of the gastrointestinal tract. This gene and its exclusive ligand, chemokine 25, are overexpressed in a variety of malignant tumors and are closely associated with tumor proliferation, apoptosis, invasion, migration and drug resistance. This gene maps to the chemokine receptor gene cluster. Multiple transcript variants encoding different isoforms have been found for this gene.
