分子别名(Synonym)
CD9,MIC3,TSPAN29,GIG2,MRP1,BTCC1,DRAP27,5H9
表达区间及表达系统(Source)
Human CD9, His Tag (CD9-H5220) is expressed from human 293 cells (HEK293). It contains AA Ser 112 - Ile 195 (Accession # P21926-1).
Predicted N-terminus: Ser 112
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 11.6 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE).
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Human CD9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
背景(Background)
CD9 antigen is also known as tetraspanin-29 (TSPAN29), 5H9 antigen, Leukocyte antigen MIC3 (MIC3), Motility-related protein, is a multi-pass membrane protein which belongs to the tetraspanin (TM4SF) family or the transmembrane 4 superfamily. CD9 is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. TSPAN29 is found on the surface of exosomes. MIC3 Involved in platelet activation and aggregation, regulates paranodal junction formation and also Involved in cell adhesion, cell motility and tumor metastasis. CD9 antigen also seems to be a key part in the egg-sperm fusion during mammalian fertilization.