【ComboX】ComboX | 标签如翼，让生物分析更快更特异

标签/标记技术作为药物发现、药物评价和治疗策略优化的有力工具和方法，在生物药研发流程中发挥着重要作用，如早期研发阶段的药物靶标鉴定、药物筛选，CMC阶段的质量控制，临床前及临床阶段的药代动力学研究等。

● 药物靶标鉴定：药物或候选药物与特定的标签结合，追踪和鉴定其在生物体内的特异性结合靶点，从而揭示药物的作用机制和生物活性，指导药物设计和优化。

● 分子相互作用研究：借助生物分子与标签的特异性结合，追踪和检测药物与受体、配体之间的相互作用，明确生物分子的功能、信号传导途径以及药物的相互作用，为药物研发提供指导。

● 通路研究及功能验证：通过将标签结合于特定的细胞信号分子上，帮助跟踪和检测药物在细胞内的位置、转运、功能、相互作用，为靶点及通路研究、药物功能验证提供依据。

● 药代动力学研究：通过将药物直接或间接与特定标签结合，跟踪和测定药物在体内的浓度分布、代谢速率和排泄途径，探寻血药浓度随时间而变化的规律，评价药物的安全性及有效性。

作为一种多功能亲和标签，链霉亲和素（Streptavidin，SA）能够稳定结合生物素，从而实现对生物素分子及生物素标记物质的检测。SA与众多荧光基团及酶偶联物标记，已广泛应用于蛋白质印迹、免疫组织化学、免疫荧光、流式细胞术和ELISA，在标签界发挥着重要的作用，堪得“花式大玩家”的称号。

✔ ACROBiosystems百普赛斯可提供一系列SA标记蛋白产品：SA-HRP，SA-APC，SA-PE，SA-FITC，SA- Texas Red，SA-AE，SA-AF488/AF555/AF647，SA-Cy5/Cy3等，另有多种SA包被板、SA磁珠产品可供选择，为您的药物研发提供有力工具。

● SA-HRP

Measure performance comparison between Acro (Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin, Cat. No. STN-NH913) and other competitive products. With tight quality control measures, Acro has better sensitivity than other brands.

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● SA-APC

5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 20 μg/mL Biotinylated Human CD19 (20-291) Protein, Fc,Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-APC (Cat. No. STN-NA113) and analyzed with FACS. APC signal was used to evaluate the binding activity (QC tested).

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● SA-PE

5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 20 μg/mL Biotinylated Human CD19 (20-291), Fc,Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-PE (ACROBiosystems, Cat. No. STN-NP119 & Brand 1 & Brand 2) and analyzed with GraphPad Prism 5. The results showed that the activity of PE-SA of ACROBiosystems is higher than other two competing brands (Routinely tested).

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● SA-FITC

5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 20 μg/mL Biotinylated Human CD19 (20-291) Protein, Fc,Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 1.25 μg/mL of Streptavidin Protein-FITC (Cat. No. STN-NF113) and analyzed with FACS. FITC signal was used to evaluate the binding activity (QC tested).

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His标签是一段含有多个组氨酸残基的多肽序列，融合到目标重组蛋白的N端或C端后，对蛋白的结构和特性几乎没有影响，是蛋白纯化和检测的常用标签之一，非常受欢迎，可谓标签界的“中流砥柱”。基于此，ACROBiosystems百普赛斯开发了针对His标签的通用型产品：Anti-His抗体和镍包被板。

● Anti-His抗体

Ant-His抗体能与His标签特异性结合，从而实现对带有His标签的目标蛋白的定位、追踪和表征。ACROBiosystems百普赛斯的Anti-His抗体产品质控严格，高纯度经MALS验证，高生物活性经ELISA验证，具有高特异性、高灵敏度、高性价比的优势，致力于加速生物药研发进程。

验证数据

高生物活性经ELISA验证

Immobilized Human BAFF Protein, Fc Tag (Cat. No. BAF-H5261) at 2 μg/mL (100 μL/well) can bind Human TACI Protein, His Tag (Cat. No. TAI-H52H3) when detected by HRP conjugated Anti-His tag Antibody (AY63), mAb (Cat. No. HIS-LY63) dilute at 1:5000(0.108μg/ml)(QC tested).

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Immobilized Human IL-4 R alpha Protein, His Tag (Cat. No. ILR-H5221) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-His Tag Antibody, Mouse IgG1 (AY63) (Cat. No. HIS-AY63) with a linear range of 0.20-3.13 ng/mL (QC tested).

● 镍包被板

镍包被板的镍包被层能够与His标签结合，从而实现对带有His标签的蛋白质的选择性捕获。经验证，ACROBiosystems百普赛斯推出镍包被板可特异性结His标签蛋白，适用于免疫/ELISA/CLIA等方法学的检测场景。

高生物活性经ELISA验证

Immobilized Cynomolgus Siglec-10, His Tag at 1 μg/mL (100 μL/well) on Nickel Coated plates, Clear, 12×8-Well Strips(Cat.No. SP-16), can bind Anti-Cynomolgus Siglec-10 Antibody with a linear range of 0.002-1.563μg/mL (QC tested).

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高结合活性与同类竞品相当

Immobilized Cynomolgus Siglec-10, His Tag at 1 μg/mL (100 μL/well) on Nickel Coated plates, Clear, 12×8-Well Strips (Cat. No. SP-16), the binding activity with Anti-Cynomolgus Siglec-10 Antibody was the similar with the other nickel plate.

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