【ActiveMax】小磁珠大能量——GMP级别CD3/CD28抗体偶联磁珠

体外诱导活化T淋巴细胞常用于细胞免疫疗法如CAR-T、TIL疗法的工业生产中，一般会利用抗CD3抗体和抗CD28抗体或CD3/CD28抗体偶联磁珠模拟体内第一和第二刺激信号过程来激活T细胞，尤其是CD3/CD28抗体偶联磁珠，具有激活作用更强、可保留更多T细胞记忆表型、T细胞耗竭更少以及临床疗效更加持久等优势，是细胞治疗药物开发过程中不可或缺的重要原料。

ACROBiosystems百普赛斯专注于支持细胞疗法相关研究，在完善的GMP质量体系下，结合细胞培养平台、磁珠偶联技术平台、流式细胞分析平台，开发出高质量的ActiveMax^® GMP级别CD3/CD28抗体偶联磁珠产品（货号：GMP-MBS001），经全方位活性验证，可高效刺激扩增T细胞，加速您的细胞治疗药物开发进程。

The human T cells were stimulated with GMP ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads (Cat. No. GMP-MBS001) for 24hrs, and the activation was assessed by measuring expression of both activation markers CD25 and CD69 expression on the T cells surface by stanning with PE labeled anti-human CD25 antibody and FITC labeled anti-human CD69 antibody respectively (QC tested).

The human T cells were labeled with carboxy fluorescein succinimidyl ester (CFSE) and stimulated with GMP ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads (Cat. No. GMP-MBS001), and then the proliferation of the T cells was assessed with CFSE dilution assay by flow cytometry on day 5 after stimulation (QC tested).

Activation of the purified human T Cells. The purified human T cells were activated using Human T cell Activation/Expansion CD3/CD28 Beads, (ACRO, Cat. No. GMP-MBS001) and Competitor-Beads respectively for 24 hours with CTS Optimizer Medium. Cells were fluorescently stained using PE labeled anti-human CD25 antibody and labeled FITC anti-human CD69 antibody and analyzed by flow cytometry.

Expansion of the human CD3+T cells. Human T cells using ACROBiosystems CD3/CD28 Beads (ACRO, Cat. No. GMP-MBS001) were expanded under two different medium, respectively. Expansion was performed for two weeks, showing that ACROBiosystems’ beads showing better proliferative abilities and comparable competitive ideas compared with competitor product.

PD-1 expression of the activated human T Cells. The purified human T cells were stimulated using Human T cell Activation/Expansion CD3/CD28 Beads at a ratio of 1:1 beads-to-cells. Cells were expanded in T cell culture medium supplemented with 4ng/mL of rhIL-2 Protein (Acrobiosystems, Cat. No. IL2-H4113). Activated T cells were expanded for up to 8 days with low PD-1 expression.

The Cell based assay shows that GMP ActiveMax® Human T cell Expansion CD3/CD28 Beads(GMP-MBS001) is stable at 37℃ for 35 days.