FACS验证,免费提供protocol
Ilia G. Denisov, et al., Nanodisc in membrane biochemistry and biophysics. Chem Rev. 2017(页面尾部可下载文献全文)
分子 | 货号 | 产品描述 | 应用 | 订购/预购 |
---|---|---|---|---|
CD20 | CD0-H52D5 | Human CD20 / MS4A1 Full Length Protein, Flag,His Tag (Detergent) |
免疫 ELISA SPR BLI |
|
CD20 | CD0-H52H3 | Human CD20 / MS4A1 Full Length Protein, His Tag (Detergent) (SPR verified) | ||
CD20 | CD0-H82E5 | Biotinylated Human CD20 / MS4A1 Full Length Protein, His,Avitag™ (Detergent) (SPR verified) | ||
CD20 | CD0-C52H8 | Cynomolgus CD20 / MS4A1 Full Length Protein, His Tag (Detergent) | ||
CD20 | CD0-C52H5 | Canine CD20 / MS4A1 Full Length Protein, His Tag (Detergent) | ||
CD20 | CD0-C52Hb | Cat CD20 / MS4A1 Full Length Protein, His Tag (Detergent) | ||
Buffer | DC-11 | 200x DDM CHS buffer | 维持膜蛋白溶解性和活性 稀释膜蛋白或抗体 在SPR或BLI检测中制备运行缓冲液 |
分子 | 货号 | 产品描述 | 应用 | 订购/预购 |
---|---|---|---|---|
CD20 | CD0-H52H1 | Human CD20 / MS4A1 Full Length Protein, His Tag (Nanodisc) |
免疫 ELISA SPR BLI 细胞实验 CAR阳性率检测 |
|
CD20 | CD0-H82E3 | Biotinylated Human CD20 / MS4A1 Full Length Protein, His,Avitag™ (Nanodisc) | ||
CD20 | CD0-HF2H5 | FITC-Labeled Human CD20 / MS4A1 Full Length Protein, His Tag (Nanodisc, SPR verified) | ||
CD20 | CD0-C52H9 | Canine CD20 / MS4A1 Full Length Protein, His Tag (Nanodisc) | ||
MSP1D1 | APO-H51H3 | Human MSP1D1 Protein, His Tag (Nanodisc) | 同型对照 | |
MSP1D1 | APO-H81Q5 | Biotinylated Human MSP1D1 Protein, His,Avitag™ (Nanodisc) |
分子 | 货号 | 产品描述 | 应用 | 订购/预购 |
---|---|---|---|---|
CD20 | CDP-H52P6 | Human CD20 Full Length Protein (VLP) |
免疫 ELISA SPR/BLI 细胞实验 CAR阳性率检测 |
|
CD20 | CDP-HF2P8 | Fluorescent Human CD20 Full Length Protein (VLP) | ||
VLP | VLP-N5213 | Virus-Like Particle (VLP) Protein Isotype Control | VLP同型对照 | |
VLP | VLP-NF2P4 | Fluorescent Virus-Like Particle (VLP) Protein Isotype Control |
A) HEK293 Cell (Full Length)
Immobilized Rituximab at 2 μg/mL (100 μL/well) can bind Human CD20 Full Length, His Tag, HEK293 (SPR verified) (Cat. No. CD0-H52H3) with a linear range of 0.4-3 ng/mL (in presence of DDM and CHS).
Protocol
B) E.coli (Extracellular)
Immobilized Rituximab at 5 μg/mL (100 μL/well) can bind Human CD20, TrxA Tag, His Tag (E.coli) with a linear range of 78-2500 ng/mL.
2e5 of CD20-CAR-293 cells transfected with anti-CD20-scFv were stained with 100 μL of 3 μg/mL of Biotinylated Human CD20 Full Length, His,Avitag (Cat. No. CD0-H82E3) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
Protocol
2e5 of CD20-CAR-293 cells transfected with anti-CD20-scFv were stained with 100 μL of 3 μg/mL of Human CD20 / MS4A1 Full Length Protein, His Tag (Nanodisc) (HEK293)(Cat. No. CD0-H52H1) and negative control protein respectively, washed and then followed by PE anti-His antibody and analyzed with FACS (QC tested).
Protocol
Immobilized Rituximab at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD20 Full Length, His,Avitag (Cat. No. CD0-H82E3) with a linear range of 0.008-0.125 μg/mL
Protocol
Immobilized Rituximab at 2 μg/mL (100 μL/well) can bind Human CD20 Full Length, His Tag (Cat. No. CD0-H52H1) with a linear range of 1-63 ng/mL
Protocol
Immobilized Rituximab at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD20 Full Length, His,Avitag (HEK293) (Cat. No. CD0-H82E5) with a linear range of 4-63 ng/mL (in presence of DDM and CHS).
Protocol
Immobilized Ofatumumab at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD20 Full Length, His,Avitag (HEK293) (Cat. No. CD0-H82E5) with a linear range of 4-63 ng/mL (in presence of DDM and CHS).
Protocol
Immobilized Rituximab at 2 μg/mL (100 μL/well) can bind Human CD20 Full Length, His Tag, HEK293 (SPR verified) (Cat. No. CD0-H52H3) with a linear range of 0.4-3 ng/mL (in presence of DDM and CHS).
Protocol
Immobilized Ofatumumab at 2 μg/mL (100 μL/well) can bind Human CD20 Full Length, His Tag, HEK293 (SPR verified) (Cat. No. CD0-H52H3) with a linear range of 0.4-6 ng/mL (in presence of DDM and CHS).
Protocol
Biotinylated Human CD20, His,Avitag (HEK293) (Cat. No. CD0-H82E5) captured on Biotin CAP-Series S Sensor Chip can bind Rituximab with an affinity constant of 1.73 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore T200).
Protocol
Human CD20 Full Length, His Tag, HEK293 (SPR verified) (Cat. No. CD0-H52H3) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind MabThera® (Rituximab) with an affinity constant of 6.21 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K).
Protocol
Authors: Lionel Rougé et al.
Journal: Science.aaz9356
Authors: Ilia G. Denisov, et al.
Journal: Chem Rev. 2017