膜杰作
Star Staining
分子别名（Synonym）
TNFRSF17,CD269,BCM,BCMA
表达区间及表达系统（Source）
Mouse BCMA, Fc Tag (BCA-M5258) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Thr 49 (Accession # O88472-1).
Predicted N-terminus: Met 1
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 32.1 kDa. The protein migrates as 36 kDa under reducing (R) condition (SDS-PAGE) due to O-linked glycosylation which has been verified by O-Glycosidase and Neuraminidase digestion.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Mouse BCMA, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Mouse BAFF, Mouse IgG2a Fc Tag (Cat. No. BAF-M5257) at 5 μg/mL (100 μL/well) can bind Mouse BCMA, Fc Tag (Cat. No. BCA-M5258) with a linear range of 2-20 ng/mL (QC tested).
Protocol
Immobilized Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) at 2 μg/mL (100 μL/well) can bind Mouse BCMA, Fc Tag (Cat. No. BCA-M5258) with a linear range of 0.3-2 ng/mL (Routinely tested).
Protocol
背景（Background）
Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.
