膜杰作
Star Staining
& Cat.No. | Items | Size （2mg） | Size（5mg X 2） |
| Particle size | 2 μm | 2 μm |
| Physical appearance | Powder mixture | Powder mixture |
| Amount of Coupled Protein | ≈1698 pmol (16.3 μg) BCMA/mg Beads | ≈1698 pmol (16.3 μg) BCMA/mg Beads |
| Binding Capacity | >267 pmol (40 μg) antibody/mg beads | >267 pmol (40 μg) antibody/mg beads |
| Formulation | PBS, pH7.4, with 10% Trehalose | PBS, pH7.4, with 10% Trehalose |
| Reconstitution | 2 mL sterile deionized water (1 mg beads/mL) | 5 mL sterile deionized water (1 mg beads/mL) |
背景（Background）
The biotinylated BCMA protein was conjugated to streptavidin magnetic beads. This pre-coupled magnetic bead product can capture the anti-BCMA antibody from various assay systems. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This Human BCMA-coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready-to-use product could greatly save your time and hassle.
表达区间及表达系统（Source）
Human BCMA protein is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54(Accession # Q02223-1).
应用说明（Application）
This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C. Once reconstitution, please aliquote and store under -70°C for no more than 3 months, and avoid repeated freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
原理（Assay Principles）
Antibody Purification: 1. Resuspend the lyophilized beads by adding the buffer of choice. 2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein. 3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads. 4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.
The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
典型数据-Typical Data
Add BCMA pre-coupling magnetic beads into each well at a concentration of 1 mg/ml. 0.1 mg beads was added to each well. Then add increasing concentrations of anti-BCMA antibody to different wells. PE-labeled anti-human IgG Fc antibody was used for detection.
Protocol
The binding curves between BCMA pre-coupling magnetic beads (Cat. No. MBS-K004) after different freeze-thaw cycles and anti-BCMA antibody. 0.1 mg of Beads (1 mg/mL, 100 μL) was washed three times and the supernatant was removed. 100 μL antibodies of the corresponding concentration (10 μg/mL-0.039 μg/mL) were added. Fluorescent labeled secondary antibody was added for detection (Routinely tested).
Protocol
