膜杰作
Star Staining
描述（Description）
The Human LAG-3 (Luc) Jurkat Reporter Cell was engineered to not only express the NFAT response element driving luciferase expressing systems, but also express the receptor full length human LAG-3 (Gene ID: 3902), which can use to evaluate the potency of LAG-3 blockade. When co-cultured with target cells expressing human MHCⅡ, the LAG-3/MHCⅡ interaction inhibits TCR signaling and NFAT-mediated luminescence. Blocking the LAG-3/MHCⅡ interaction by anti-LAG-3 antibodies releases the inhibitory signals and results in TCR activation and NFAT-mediated luminescence.
应用说明（Application）
Screen for anti-human LAG-3 antibody.
生长特性（Growth Properties）
Suspension
筛选标记（Selection Marker）
Puromycin (5 μg/mL) + Hygromycin (20 μg/mL)
培养基（Culture Medium）
RPMI-1640 + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Expression analysis of human LAG-3 on Human LAG-3 (Luc) Jurkat Reporter Cell by FACS.
Human LAG-3 (Luc) Jurkat Reporter Cell or negative control cell were stained with PE-labeled anti-human LAG-3 antibody.
Protocol
Application
Blocking activity of anti-human LAG-3 antibody.
This reporter cell was incubated with serial dilutions of antibodies in the presence of target cells expressing human MHCⅡ. The EC50 of anti-human LAG-3 antibody was approximately 0.11 μg/mL.
Protocol
Passage Stability
Passage stability analysis by anti-human LAG-3 antibody stimulation.
The continuously growing Human LAG-3 (Luc) Jurkat Reporter Cell was stimulated with serial dilutions of antibodies in the presence of target cells expressing human MHCⅡ. Anti-human LAG-3 antibody stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 14-38.
Protocol
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背景（Background）
Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily. As a CD4 homologue, LAG3 expressed on the surface of activated conventional T cells and regulatory T (Treg) cells. In conventional T cells, LAG-3 acts as an inhibitory receptor of T cell inflammation.
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
