膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) is a monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.
应用（Application）
ELISA (Evaluation of levels of CD3 epsilon protein).
Flow Cytometry (Evaluation of an epitope within the extracellular domain of CD3 epsilon protein).
克隆号（Clone）
SP34-2
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1/kappa
特异性（Specificity）
This product is a specific antibody specifically reacts with CD3 epsilon protein.
种属反应性（Reactivity）
Human
免疫原（Immunogen）
Purified Human CD3ε Protein.
偶联（Conjugate）
Unconjugated
同型对照（Isotype Control）
The Isotype control is sold separately and you can search for Cat. No. DNP-M1A1 for product information.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
活性（Bioactivity）-ELISA
Immobilized Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) with a linear range of 0.4-6 ng/mL (QC tested).
Protocol
活性（Bioactivity）-FACS
2e5 of Jurkat cells were stained with 100 μL of 10 μg/mL of Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) and isotype control antibody respectively, washed and then followed by PE-anti mouse IgG1 antibody and analyzed with FACS (Routinely tested).
Protocol
背景（Background）
CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
