ActiveMax® Human CD19 μBeads, premium grade (for cells) DMF

背景（Background）

B-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I  membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. As on T cells, several surface molecules form the antigen receptor and form a complex on B lymphocytes. The (almost) B cell-specific CD19 phosphoglycoprotein is one of these molecules. The others are CD21 and CD81. These surface immunoglobulin (sIg)-associated molecules facilitate signal transduction. On living B cells, anti-immunoglobulin antibody mimicking exogenous antigen causes CD19 to bind to sIg and internalize with it. The reverse process has not been demonstrated, suggesting that formation of this receptor complex is antigen-induced. This molecular association has been confirmed by chemical studies. Mutations in CD19 are associated with severe immunodeficiency syndromes characterized by diminished antibody production. CD19 has been shown to interact with: CD81, CD82, Complement receptor 2, and VAV2.

表达区间及表达系统（Source）

ActiveMax® Human CD19 μBeads, premium grade (for cells) are uniform, superparamagnetic beads of 5.5 µm with streptavidin on its surface and coupled with biotinylated Human CD19 Protein, expressed from human 293 cells (HEK293) and contains AA Pro 20 - Lys 291 (Accession # P15391-1).

应用说明（Application）

ActiveMax® Human CD19 μBeads, premium grade (for cells) are designed to stimulate in vitro CD19-specific CAR-T cells or UCAR-T cells, similar to the tumor cell lines that express human CD19 antigen. It can be used as follows:
Evaluating the characteristics of CAR-T cells or UCAR-T cells.
In vitro expansion of CD19-specific CAR-T cells or UCAR-T cells.
In vitro enrichment of CD19-specific CAR-T cells or UCAR-T cells.

重构方法（Reconstitution）

See Certificate of Analysis (CoA) for detailed instruction.

存储（Storage）

This product is stable in storage under the following conditions: -20˚C for 12 months in lyophilized state. -70°C for 3 months under sterile conditions after reconstitution.

Please avoid repeated freeze-thaw cycles after reconstitution. Immediate use after reconstitution is highly recommended.

无菌（Sterility）

Negative

内毒素（Endotoxin）

Less than 0.002 EU per μg by the LAL method.

注意事项（Important Note）

This product is for research use only and not intended for therapeutic or in vivo diagnostic use.

制剂（Formulation）

Please contact us for detailed information.

Contact us for customized product form or formulation.