膜杰作
Star Staining
分子别名（Synonym）
DDPAC,FTDP-17,MAPT,MSTD,MTBT1,Tau,PHF-tau,TAU
表达区间及表达系统（Source）
Human Tau-441 GSK-3beta-phosphorylated protein, His Tag (TAU-H5147) is expressed from E. coli cells. It contains AA Met 1 - Leu 441 (Accession # P10636-8).
Predicted N-terminus: Met
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 47.9 kDa. The protein migrates as 45-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
电泳（SDS-PAGE）
Human Tau-441 GSK-3beta-phosphorylated protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human Tau-441 GSK-3beta-phosphorylated protein, His Tag (Cat. No. TAU-H5147) at 1 μg/mL (100 μL/well) can bind Anti-Phospho-Tau (Thr181) Monoclonal Antibody, Mouse IgG with a linear range of 1-31 ng/mL (QC tested).
Protocol
Immobilized Human Tau-441 GSK-3beta-phosphorylated protein, His Tag (Cat. No. TAU-H5147) at 1 μg/mL (100 μL/well) can bind Anti-Tau Antibody with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
质谱（Mass Spectrometry）
Mass spectrometry reveals 8 phosphorylation sites on E.coli expressed Human Tau-441 GSK-3beta-phosphorylated protein, His Tag (Cat. No. TAU-H5147) as shown on the modified phosphorylation PTM spectrum. The protein sequence coverage was 40% (Routinely tested).
WB验证（Western Blot）
Western blot was performed by loading Human Tau-441 GSK-3beta-phosphorylated protein, His Tag (Cat. No. TAU-H5147): 400ng, and it was electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel. Human Tau 441 GSK-3beta-phosphorylated protein, His Tag (Cat. No. TAU-H5147) were transferred onto a pvdf membrane by Tanon Trans-Blot. Anti-Phospho-Tau (Thr181) Monoclonal Antibody is primary antibody (1 μg/mL) and (MFC) Peroxidase Affini Pure Goat Anti-Mouse IgG, Fcγ Fragment Specific (minXHu, Bov, HrSrProt (1:1500)) is second antibody detected by chemiluminescence with using the iBright 1500 (Chemiluminescent detection was performed using BeyoECL Plus).
背景（Background）
Tau is a microtubule-associated protein, which encodes by the MAPT gene that located on chromosome 17q21. Tau Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. Hyperphosphorylation of the tau protein (tau inclusions, pTau) can result in the self-assembly of tangles of paired helical filaments and straight filaments, which are involved in the pathogenesis of Alzheimer's disease, frontotemporal dementia, and other tauopathies.
