抗体来源(Source)
Monoclonal Anti-HA (Influenza A/Darwin/9/2021 (H3N2)) Antibody, Mouse IgG1 (1D6) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.
克隆号(Clone)
1D6
种属(Species)
Mouse
亚型(Isotype)
Mouse IgG1 | Mouse Kappa
偶联(Conjugate)
Unconjugated
抗体类型(Antibody Type)
Hybridoma Monoclonal
种属反应性(Reactivity)
Virus
免疫原(Immunogen)
Recombinant Influenza A [A/Darwin/9/2021 (H3N2)] HA Protein is expressed from human 293 cells.
特异性(Specificity)
Specifically recognizes HA (Influenza A/Darwin/9/2021 (H3N2)).
应用(Application)
Application | Recommended Usage |
ELISA | 0.4-100 ng/mL |
纯度(Purity)
>95% as determined by SDS-PAGE.
纯化(Purification)
Protein A purified/ Protein G purified
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.0 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
交叉验证(Cross Verification)
This product No cross-reactivity in ELISA with
Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H8).
Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7).
Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag (Cat. No. HA1-V5229).
Influenza A (Vietnam/1194/2004(H5N1)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H9).
Influenza A (Guangdong/18SF020(H5N6)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA6-V52H3).
Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3).
Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3).
Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4).
电泳(SDS-PAGE)
Monoclonal Anti-HA (Influenza A/Darwin/9/2021 (H3N2)) Antibody, Mouse IgG1 (1D6) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性(Bioactivity)-ELISA
Immobilized Influenza A [A/Darwin/9/2021 (H3N2)] Hemagglutinin (HA) Protein, His Tag (MALS verified) (Cat. No. HA2-V52H6) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-HA (Influenza A/Darwin/9/2021 (H3N2)) Antibody, Mouse IgG1 (1D6)(Cat. No. HA2-Y196) with a linear range of 0.195-6.25 ng/mL (QC tested).
Protocol
Immobilized Influenza A [A/Darwin/9/2021 (H3N2)] Hemagglutinin (HA) Protein, His Tag (MALS verified) (Cat. No. HA2-V52H6) can bind Monoclonal Anti-HA (Influenza A/Darwin/9/2021 (H3N2)) Antibody, Mouse IgG1 (1D6)(Cat. No. HA2-Y196). The antibody does not bind Influenza A [A/Bangkok/1/1979 (H3N2)] Hemagglutinin (HA) Protein, His Tag (MALS verified)(Cat. No. HA2-V52H3), Influenza A [A/Wisconsin/588/2019 (H1N1)] Hemagglutinin (HA) Protein, His Tag (MALS verified)(Cat. No. HA1-V52H3), Influenza A [A/Darwin/6/2021 (H3N2)] Hemagglutinin (HA) Protein, His Tag (MALS verified)(Cat. No. HA2-V52H5), Influenza A [Sydney/5/2021 (H1N1)] Hemagglutinin (HA) Protein, His Tag (MALS verified)(Cat. No. HA1-V52H4), Influenza B [Austria/1359417/2021 (B/Victoria lineage)] Hemagglutinin (HA) Protein, His Tag(Cat. No. HAE-V52H3), Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin 1 (HA1) Protein, His Tag (MALS verified)(Cat. No. HA1-V52H5), Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA) Protein, His Tag (MALS verified)(Cat. No. HA2-V52H7) and Influenza A [A/Victoria/2570/2019] Hemagglutinin (HA) Protein, His Tag (MALS verified)(Cat. No. HA1-V52H6). (Routinely tested).
Protocol
背景(Background)
Influenza, commonly known as 'the flu', is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses. The virus is divided into three main types (Influenzavirus A, Influenzavirus B, and Influenzavirus C), which are distinguished by differences in two major internal proteins (hemagglutinin (HA) and neuraminidase (NA), which are the most important targets for the immune system. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle which makes it a great target for vaccine studies.