膜杰作
Star Staining
分子别名（Synonym）
STEAP1, PRSS24, STEAP
表达区间及表达系统（Source）
Human STEAP1 Full Length Protein, Twin-Strep,His Tag (ST1-H5283) is expressed from human 293 cells (HEK293). It contains AA Glu 2 - Leu 339 (Accession # Q9UHE8-1).
Predicted N-terminus: Met
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a twin strep tag at the N-terminus and a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 44.6 kDa. The protein migrates as 40-42 kDa and 80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to Glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>85% as determined by SDS-PAGE.
制剂（Formulation）
This product is not suitable for cell based experiments due to cytotoxicity of Digitonin.
Digitonin is INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove Digitonin.
Digitonin buffer (DN-12) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, Digitonin, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
Human STEAP1 Full Length Protein, Twin-Strep,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human STEAP1 Full Length Protein, Twin-Strep,His Tag (Cat. No. ST1-H5283) at 5 μg/mL (100 μL/well) on a Nickel Coated plate can bind Anti-STEAP1 Antibody with a linear range of 0.156-2.5 μg/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Anti-STEAP1 Antibody captured on Protein A Chip can bind Human STEAP1 Full Length Protein, Twin-Strep,His Tag (Cat. No. ST1-H5283) with an affinity constant of 2.95 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
Six-transmembrane epithelial antigen of the prostate 1 (STEAP1) is an integral membrane protein that is highly up-regulated on the cell surface of several human cancers, making it a promising therapeutic target to manage these diseases. It shares sequence homology with three enzymes (STEAP2-STEAP4) that catalyze the DPH-dependent reduction of iron(III). Taking into account its high specificity and overexpression in human cancers, STEAP1 is nowadays a promising candidate to be imposed as a therapeutic target.
