膜杰作
Star Staining
描述（Description）
The HEK293/Human GLP-1R Stable Cell Line was engineered to express the receptor full length human GLP-1R (Gene ID: 2740), with different levels of GLP-1R expression (High, Medium, Low). Surface expression of human GLP-1R was confirmed by flow cytometry.
应用说明（Application）
• Useful for cell-based GLP-1R binding assay
• Screen for human GLP-1R agonists based on cAMP accumulation assay
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Hygromycin (20 μg/mL)
培养基（Culture Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Expression analysis of human GLP-1R on HEK293/Human GLP-1R Stable Cell Line by FACS.
Cell surface staining using PE-labeled anti-human GLP-1R antibody was performed on HEK293/Human GLP-1R Stable Cell Line with different expression levels: HEK293/Human GLP-1R Stable Cell Line (Low Expression); HEK293/Human GLP-1R Stable Cell Line (Medium Expression); HEK293/Human GLP-1R Stable Cell Line (High Expression).
Protocol
Application
cAMP accumulation assay for human GLP-1R agonist screening.
HEK293/Human GLP-1R Stable Cell Line (Low Expression), HEK293/Human GLP-1R Stable Cell Line (Medium Expression) and HEK293/Human GLP-1R Stable Cell Line (High Expression) were stimulated with Tirzepatide, respectively. The EC50 of Tirzepatide on HEK293/Human GLP-1R Stable Cell Line (Low Expression) was approximately 0.00136 μM.
Protocol
Passage Stability
Passage stability analysis of receptors expression by FACS.
Flow cytometry surface staining of human GLP-1R on HEK293/Human GLP-1R Stable Cell Line (Low Expression) demonstrates consistent mean fluorescent intensity across passage 8-22.
Protocol
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背景（Background）
This gene encodes a 7-transmembrane protein that functions as a receptor for glucagon-like peptide 1 (GLP-1) hormone, which stimulates glucose-induced insulin secretion. This receptor, which functions at the cell surface, becomes internalized in response to GLP-1 and GLP-1 analogs, and it plays an important role in the signaling cascades leading to insulin secretion. It also displays neuroprotective effects in animal models. Polymorphisms in this gene are associated with diabetes. The protein is an important drug target for the treatment of type 2 diabetes and stroke.
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
