膜杰作
Star Staining
分子别名（Synonym）
betaKlotho,beta-klotho,BKL,KLB,klotho beta like,klotho beta-like protein
表达区间及表达系统（Source）
Human Klotho beta Protein, His Tag (KLB-H52H4) is expressed from human 293 cells (HEK293). It contains AA Met 30 - Leu 997 (Accession # Q86Z14-1).
Predicted N-terminus: Met 30
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 112.9 kDa. The protein migrates as 65 kDa,95 kDa and 130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Klotho beta Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human Klotho beta Protein, His Tag (Cat. No. KLB-H52H4) at 10 μg/mL (100 μL/well) can bind Human FGF19, Fc Tag (Cat. No. FG9-H5253) with a linear range of 0.005-0.156 μg/mL (QC tested).
Protocol
背景（Background）
KLB (Klotho Beta) is a Protein Coding gene. Among its related pathways are RET signaling and HIV Life Cycle. GO annotations related to this gene include hydrolase activity, hydrolyzing O-glycosyl compounds and fibroblast growth factor binding. An important paralog of this gene is KL. Klotho Beta is a regulator in multiple metabolic processes, while its role in cancer remains unclear. We found the expression of βKlotho was down-regulated in human hepatocellular carcinoma tissues compared with that in paired adjacent non-tumourous liver tissues. Hepatoma cells also showed decreased expression of βKlotho compared with normal hepatocyte cells. Reintroduction of βKlotho into hepatoma cells inhibited their proliferation.
