膜杰作
Star Staining
表达区间及表达系统（Source）
APC-Labeled Mouse H-2Kd&B2M&RSV F glycoprotein (KYKNAVTEL) Tetramer Protein (H2N-MA2H4) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Thr 305 (H-2Kd) & Ile 21 - Met 119 (B2M) & KYKNAVTEL peptide (Accession # P01902 (H-2Kd) & P01887 (B2M) & KYKNAVTEL).
Predicted N-terminus: Gly 22 & Ile 21
Request for sequence
蛋白结构（Molecular Characterization）
APC-Labeled Mouse H-2Kd&B2M&RSV F glycoprotein (KYKNAVTEL) Tetramer Protein is assembled by biotinylated monomer and APC-labeled streptavidin.
Biotinylated Mouse H-2Kd&B2M&RSV F glycoprotein (KYKNAVTEL) Complex Protein is produced by co-expression of H-2Kd and B2M loaded with RSV F glycoprotein peptide. Biotinylated Mouse H-2Kd&B2M&RSV F glycoprotein (KYKNAVTEL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
偶联（Conjugate）
APC
Excitation Wavelength: 640 nm
Emission Wavelength: 661 nm
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
Respiratory syncytial virus (RSV) is a major cause of respiratory disease in infants and the elderly. The two major glycoproteins on the surface of the RSV virion, the attachment glycoprotein (G) and the fusion glycoprotein (F), control the initial phases of infection. G targets the ciliated cells of the airways, and F causes the virion membrane to fuse with the target cell membrane. The F protein is the major target for antiviral drug development, and both G and F glycoproteins are the antigens targeted by neutralizing antibodies induced by infection.The Mouse H-2Kb&B2M&RSV F glycoprotein (KYKVTEL) Tetramer Protein is a complex of H-2Kb of the MHC Class I, B2M, and KYKVTEL peptide of the RSV F glycoprotein.
